The chamber is called the upper chamber, and the culture plate is called the lower chamber. The upper chamber contains the upper culture medium, and the lower chamber contains the lower culture medium. The upper and lower culture medium are separated by polycarbonate film.??The cells were planted in the upper chamber. Due to the permeability of the polycarbonate membrane, the components in the lower culture medium could affect the cells in the upper chamber, so that the effects of the components in the lower culture medium on the growth and movement of cells could be studied.??