Introduction

Western Blot method uses polyacrylamide gel electrophoresis. The detected object is protein, the "probe" is antibody, and the "color" is labeled secondary antibody. Protein samples separated by PAGE (polyacrylamide gel electrophoresis) are transferred to solid phase carriers (e.g. nitrocellulose films), which adsorb proteins in a non-covalent form and retain the type of peptides separated by electrophoresis and their biological activity unchanged. The protein or polypeptide on the solid phase carrier is used as antigen to react with the corresponding antibody, and then react with the enzyme or isotope labeled second antibody. The protein components expressed by specific target genes isolated by electrophoresis can be detected by substrate color or autoradiography. The technique is also widely used to detect protein level expression.

1 Methods

1.1 Reagent preparation

1.2 Sample preparation

1.3 Content determination

1.4 The immune response

1.5 chemiluminescence

1.6 Gel image analysis