Introduction

Bacterial genome has the characteristics of relatively small, high repeat sequence and easy mutation, so it is necessary to sequence the whole genome of microorganisms. Bacterial de novo sequencing has replaced the traditional methods and become an important tool to study the evolutionary genetic mechanism and key functional genes of bacteria. At present, bacterial whole genome sequencing can obtain 0 gap completion map by using third-generation sequencing. Accurately detect structural variation and movable components. The genome-wide epigenetic maps of 6mA and 4mc were obtained.

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1. Bioinformatics analysis

1.1 Raw data quality assessment

1.2 Genome assembly

1.3 KEGG, eggNOG, GO

1.4 Phylogenetic tree

1.5 Gene family analysis

1.6 Subcellular localization

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2. Sample requirements

2.1 Total DNA > 10 μg

2.2 Concentration > 100 ng/μL

2.3 260/280 at 1.8~2.2