RNA is synthesized in the direction of 5' to 3' end, using the antisense strand of DNA double strand as template, under the catalysis of RNA polymerase, using four nucleosides of triphosphate as raw materials, according to the principle of base pairing, through the formation of phosphate diester bond, without the involvement of primers, synthetic RNA with the same sequence as DNA coding chain. The basic process of transcription consists of the following four parts: template recognition, initiation of transcription, passage through promoters, extension of transcription, and termination of transcription.
1 RNA types
agomir and antagomir
Terminal modified RNA:?Thiol, Amino, biotin, phos, C9, C18 ?
Fluorescent modified RNA:?FAM, HEX, Cy3, Cy5, TET, TAMRA?
Specially modified RNA:?DBCO, Desthiobiotin, Cy5.5, Cy7, Hexynyl, BHQ1, BHQ2, BHQ3 ?
Photosensitive modified RNA:?dT-NPOM PL ??
Digoxin modified RNA:?ATTO
Some modified RNA:?2'OME, dI, dU, rI,?2'F, 2'MOE, LNA,?m6A, m5C, m5U?
2 RNA Service
The length of RNA ranged from 4 to 80 nt
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