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      3. RNA合成定制


        RNA is synthesized in the direction of 5' to 3' end, using the antisense strand of DNA double strand as template, under the catalysis of RNA polymerase, using four nucleosides of triphosphate as raw materials, according to the principle of base pairing, through the formation of phosphate diester bond, without the involvement of primers, synthetic RNA with the same sequence as DNA coding chain. The basic process of transcription consists of the following four parts: template recognition, initiation of transcription, passage through promoters, extension of transcription, and termination of transcription.

        1 RNA types


        Single-stranded RNA(ssRNA)

        agomir and antagomir

        Terminal modified RNA:?Thiol, Amino, biotin, phos, C9, C18 ?

        Fluorescent modified RNA:?FAM, HEX, Cy3, Cy5, TET, TAMRA?

        Specially modified RNA:?DBCO, Desthiobiotin, Cy5.5, Cy7, Hexynyl, BHQ1, BHQ2, BHQ3 ?

        Photosensitive modified RNA:?dT-NPOM PL ??

        Digoxin modified RNA:?ATTO

        Some modified RNA:?2'OME, dI, dU, rI,?2'F, 2'MOE, LNA,?m6A, m5C, m5U?

        2 RNA Service

        The length of RNA ranged from 4 to 80 nt

        The synthesized RNA was purified by HPLC??

        3 Order

        To order, please email technical support at biotech.service@wushanbio.com

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